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PLU‐1 nuclear protein, which is upregulated in breast cancer, shows restricted expression in normal human adult tissues: A new cancer/testis antigen?
Author(s) -
Barrett Angela,
Madsen Bente,
Copier John,
Lu Pei Juan,
Cooper Lucienne,
Scibetta Angelo G.,
Burchell Joy,
TaylorPapadimitriou Joyce
Publication year - 2002
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.10644
Subject(s) - antiserum , breast cancer , biology , antigen , polyclonal antibodies , cancer , male breast cancer , western blot , immunocytochemistry , gene , complementary dna , nuclear localization sequence , cancer research , microbiology and biotechnology , immunology , endocrinology , genetics
The PLU‐1 gene is expressed at the level of message in breast cancers and breast cancer cell lines and shows restricted expression in normal adult tissues with the exception of testis. The predicted protein sequence contains several domains, including the PLU domain, which is shared by other proteins involved in transcription and/or development. We have developed a polyclonal antiserum to a C‐terminal fragment of the PLU‐1 protein, which shows little homology to other family members. Immunohistochemic analysis with the antiserum α‐PLU‐1C confirmed the nuclear localisation of PLU‐1. α‐PLU‐1C also reacted with the mouse homologue of PLU‐1 (mPlu‐1) but not with the closest family member, RBP2. Using Western blot analysis, PLU‐1 was shown to be well expressed in breast cancers and breast cancer cell lines, while it was not detected in a range of normal adult tissues. Our results suggest that the PLU‐1 protein may belong to the class of testis/cancer antigens. © 2002 Wiley‐Liss, Inc.