Premium
Gene expression analysis of human thymoma correlates with tumor stage
Author(s) -
Sasaki Hidefumi,
Ide Nobuyuki,
Fukai Ichiro,
Kiriyama Masanobu,
Yamakawa Yosuke,
Fujii Yoshitaka
Publication year - 2002
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.10624
Subject(s) - thymoma , gene expression , biology , gene , in vivo , stage (stratigraphy) , oligonucleotide , microarray , pathology , gene expression profiling , microarray analysis techniques , cancer research , microbiology and biotechnology , genetics , immunology , medicine , paleontology
Thymoma is one of the most common solid tumors in the mediastinum. The recent development of high‐density oligonucleotide arrays provides a unique opportunity to generate gene expression profiles of cells from various stages of tumor progression as it occurs in actual neoplastic tissues. We used oligonucleotide arrays to monitor in vivo gene expression levels in early‐ (stage I or II) and late‐ (stage IVa) stage thymoma tissues in 36 patients. These in vivo gene expression profiles were verified by real‐time quantitative RT‐PCR using LightCycler. Using both methods, 2 candidate genes were identified that were more highly expressed in advanced‐stage thymomas. One was a well‐known gene , c‐JUN, and another was an unknown gene, AL050002. AL050002 expression, but not c‐JUN expression, was also correlated with the WHO classification (type B3 vs. type B1, B2 or AB). The combined use of oligonucleotide microarray and real‐time RT‐PCR analyses provides a powerful new approach to elucidate the in vivo molecular events correlated with tumor stage of thymoma. © 2002 Wiley‐Liss, Inc.