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A novel gene, NMES1, downregulated in human esophageal squamous cell carcinoma
Author(s) -
Zhou Jin,
Wang Huixin,
Lu Ailing,
Hu Gengxi,
Luo Aiping,
Ding Fang,
Zhang Jian,
Wang Xiuqin,
Wu Min,
Liu Zhihua
Publication year - 2002
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.10600
Subject(s) - carcinogenesis , biology , esophagus , complementary dna , in situ hybridization , gene , gene expression , immunohistochemistry , microbiology and biotechnology , northern blot , pathology , western blot , downregulation and upregulation , esophageal cancer , epidermoid carcinoma , cancer , cancer research , genetics , medicine , anatomy , immunology
To isolate genes with different expression levels in human esophageal SCC, SSH and reverse Northern were performed between cancer tissue and its normal counterpart. Among the differentially expressed genes identified, we report here cDNA corresponding to a 0.88 kb mRNA ( NMES1 ), whose expression was observed in all 36 adjacent normal esophageal mucosae, while 31 (86%) matched cancer tissues showed a marked reduction or complete lack of its expression. Sequence analysis of its full‐length cDNA revealed a gene encoding a predicted polypeptide of 9 kDa. Northern blot showed that NMES1 was distributed mainly in the alimentary tract. The gene was mapped to 15q21.1 by screening the GenBridge 4 RH panel. Immunohistochemistry confirmed the downregulation of NMES1 in esophageal SCC at the protein level and showed that it is a nuclear protein. In situ hybridization revealed its different expression during mouse embryonic development, especially in bone, brain, stomach and intestine. The negative correlation of NMES1 expression with esophageal oncogenesis suggests its suppressive role in tumorigenesis of the esophagus, while the precise function of NMES1 still needs further investigation. © 2002 Wiley‐Liss, Inc.