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Non‐invasive molecular detection of bladder cancer recurrence
Author(s) -
Amira Najla,
Mourah Samia,
Rozet François,
Teillac Pierre,
Fiet Jean,
Aubin Philippe,
Cortesse Ariane,
Desgrandchamps François,
Le Duc Alain,
Cussenot Olivier,
Soliman Hany
Publication year - 2002
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.10561
Subject(s) - cystoscopy , medicine , bladder cancer , cystectomy , microsatellite instability , urine , urinary bladder , urology , loss of heterozygosity , transitional cell carcinoma , bladder neoplasm , microsatellite , pathology , cancer , urinary system , biology , allele , biochemistry , gene
Transitional cell carcinoma (TCC) is the most common bladder tumor and ≈90% of bladder TCC are superficial at initial diagnosis. High recurrence rate and possible progression to muscle invasive disease that is eventually indicated for radical cystectomy are established features of these tumors. Therefore, reliable predictors of tumor recurrence are of critical importance for management of superficial bladder TCC. Successful molecular diagnosis of bladder cancer by detecting genetic lesions: loss of heterozygosity (LOH) or microsatellite instability (MSI) in cells exfoliated in urine has been reported by several groups including ours. The aim of our study was to evaluate the predictive potential of microsatellite analysis of cells exfoliated in urine in the detection of superficial bladder TCC recurrence. We studied 47 Caucasian patients with confirmed superficial bladder TCC (37 pTa, 10 pT1) at initial diagnosis. Blood samples were obtained once from every patient whereas urine samples were collected before each cystoscopy (initial and follow‐up). Matched DNAs from blood and urine were subjected to microsatellite analysis in a blinded fashion. The follow‐up period ranged 12–48 months after tumor resection. Microsatellite analysis correctly identified 94% (44/47) of primary tumors and 92% (12/13) of tumor recurrences. Interestingly enough, 75% (9/12) of tumor recurrences were molecularly detected 1–9 months before cystoscopic evidence of recurrent disease. This study demonstrated clearly that not only urine microsatellite analysis reliably detected superficial bladder tumors, but also was a reliable test for detecting and predicting tumor recurrence in Caucasian patients. These results warrant multicenter randomized trials. © 2002 Wiley‐Liss, Inc.