Premium
CT7 (MAGE‐C1) antigen expression in normal and neoplastic tissues
Author(s) -
Jungbluth Achim A.,
Chen YaoTseng,
Busam Klaus J.,
Coplan Keren,
Kolb Denise,
Iversen Kristin,
Williamson Barbara,
van Landeghem Frank K.H.,
Stockert Elisabeth,
Old Lloyd J.
Publication year - 2002
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.10416
Subject(s) - immunohistochemistry , antigen , immunostaining , biology , monoclonal antibody , pathology , microbiology and biotechnology , antibody , medicine , immunology
CT7 (MAGE‐C1) is a member of the cancer testis (CT) antigen family. The present study describes the generation of CT7‐33, a monoclonal antibody (MAb) to CT7, and the preliminary protein expression analysis of CT7 in normal tissues and in a limited number of neoplastic lesions. CT7‐33 was effective in frozen as well as formalin‐fixed, paraffin‐embedded tissues, and immunohistochemistry/reverse transcriptase polymerase chain reaction (RT‐PCR) co‐typing demonstrated antibody specificity. CT7‐33 immunoreactivity in normal adult tissues is restricted to testicular germ cells. In neoplastic lesions, CT7‐33 immunostaining is confined to tumor cells, and the frequency of CT7 protein expression mostly parallels previous mRNA analyses. Whereas colorectal and renal cell carcinomas, as well as sarcomas, exhibit poor or no CT7‐33 staining, carcinomas of the mammary gland and ovary, nonsmall cell lung carcinoma and metastatic melanomas exhibit a high incidence of CT7 protein expression. However, as seen in previous analyses of other CT antigens, the expression pattern is mostly heterogeneous, and tumors with more than 50% of tumor staining are only infrequently encountered. In summary, our study presents a new serologic reagent for the analysis of CT7 on a protein level and confirms what is known with regard to the expression pattern of other CT antigens in tumors: frequent heterogeneity of antigen expression. © 2002 Wiley‐Liss, Inc.