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Assessment of genomic instability in breast cancer and uveal melanoma by random amplified polymorphic DNA analysis
Author(s) -
Papadopoulos Sarantos,
Benter Thomas,
Anastassiou Gerasimos,
Pape Michael,
Gerhard Schaller,
Bornfeld Norbert,
Ludwig WolfDieter,
Dörken Bernd
Publication year - 2002
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.10297
Subject(s) - breast cancer , cancer , melanoma , rapd , genomic dna , biology , dna , genome instability , genetics , dna profiling , microbiology and biotechnology , cancer research , dna damage , medicine , population , environmental health , genetic diversity
Some types of cancer have been associated with abnormal DNA fingerprinting. We used random amplified polymorphic DNA (RAPD) to generate fingerprints that detect genomic alterations in human breast cancer. Primers were designed by choosing sequences involved in the development of DNA mutations. Seventeen primers in 44 different combinations were used to screen a total of 6 breast cancer DNA/normal DNA pairs and 6 uveal melanoma DNA/normal DNA pairs. Forty‐five percent of these combinations reliably detected quantitative differences in the breast cancer pairs, while only 18% of these combinations detected differences in the uveal melanoma pairs. Fourteen (32%) and 12 (27%) primers generated a smear or did not produce any band patterns in the first and second cases, respectively. Taking into account the ability of RAPD to screen the whole genome, our results suggest that the genomic damage in breast cancer is significantly higher than in uveal melanoma. Our study confirms other reports that the molecular karyotypes produced with random priming, called amplotypes, are very useful for assessing genomic damage in cancer. © 2002 Wiley‐Liss, Inc.