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Spontaneous hybridization of macrophages and Meth A sarcoma cells
Author(s) -
Busund LillTove R.,
Killie Mette K.,
Bartnes Kristian,
Olsen Randi,
Seljelid Rolf
Publication year - 2002
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.10249
Subject(s) - biology , macrophage , hybrid , meth , microbiology and biotechnology , in vitro , flow cytometry , ploidy , fluorescence in situ hybridization , chemistry , chromosome , genetics , gene , botany , monomer , organic chemistry , acrylate , polymer
We present evidence of hybridization between Meth A sarcoma cells and syngeneic as well as semigeneic peritoneal macrophages. The resultant hybrids are characterized by morphology, membrane markers, ploidy, chromosomal content and functional features. Briefly, after a few days of coculture, cells appeared with morphology intermediate between the 2 original cell types. Typical macrophage surface molecules appeared in the hybrids. Meth A cells were labeled with red fluorescence and macrophages with green fluorescence. After 4 days in vitro , hybrids with yellow fluorescence appeared. Macrophages from BALB.K mice (H‐2 K k ) were cocultivated with Meth A cells from BALB/c mice (H‐2 K d ). The semigeneic hybrids displayed both specificities, as demonstrated by flow cytometry. The hybrids appeared moderately phagocytic, less so than the macrophages and markedly more so than the essentially nonphagocytic Meth A cells. The hybrids had a mean number of 76 chromosomes, as opposed to 53 in the Meth A cells and 40 in the macrophages. The macrophage DNA index was set at 1; Meth A cells were found to have an index of 1.6 in G1 phase, and the hybrids had a 2.6 index. The hybrids grew more slowly in vitro than Meth A cells, but grew faster in vivo . © 2002 Wiley‐Liss, Inc.