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Clonal chromosomal aberrations in simian virus 40‐transfected human thyroid cells and in derived tumors developed after in vitro irradiation
Author(s) -
Zitzelsberger Horst,
Bruch Jochen,
Smida Jan,
Hieber Ludwig,
Peddie Clare M.,
Bryant Peter E.,
Riches Andrew C.,
Fung Jingly,
Weier HeinzUlrich G.,
Bauchinger Manfred
Publication year - 2001
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.1015
Subject(s) - biology , comparative genomic hybridization , karyotype , thyroid , chromosome , cell culture , neoplastic transformation , cancer research , microbiology and biotechnology , carcinogenesis , genetics , gene
Abstract In vitro model cell systems are important tools for studying mechanisms of radiation‐induced neoplastic transformation of human epithelial cells. In our study, the human thyroid epithelial cell line HTori‐3 was analyzed cytogenetically following exposure to different doses of α‐ and γ‐irradiation and subsequent tumor formation in athymic nude mice. Combining results from G‐banding, comparative genomic hybridization, and spectral karyotyping, chromosome abnormalities could be depicted in the parental line HTori‐3 and in nine different HTori lines established from the developed tumors. A number of chromosomal aberrations were found to be characteristic for simian virus 40 immortalization and/or radiation‐induced transformation of human thyroid epithelial cells. Common chromosomal changes in cell lines originating from different irradiation experiments were loss of 8q23 and 13cen‐q21 as well as gain of 1q32‐qter and 2q11.2‐q14.1. By comparison of chromosomal aberrations in cell lines exhibiting a different tumorigenic behavior, cytogenetic markers important for the tumorigenic process were studied. It appeared that deletions on chromosomes 9q32‐q34 and 7q21‐q31 as well as an increased copy number of chromosome 20 were important for the tumorigenic phenotype. A comparative breakpoint analysis of the marker chromosomes found and those observed in radiation‐induced childhood thyroid tumors from Belarus revealed a coincidence for a number of chromosome bands. Thus, the data support the usefulness of the established cell system as an in vitro model to study important steps during radiation‐induced malignant transformation in human thyroid cells. © 2001 Wiley‐Liss, Inc.