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Inhibition of tumor growth and metastatic spreading by overexpression of inter‐alpha‐trypsin inhibitor family chains
Author(s) -
Paris Sébastien,
Sesboüé Richard,
Delpech Bertrand,
Chauzy Claude,
Thiberville Luc,
Martin JeanPierre,
Frébourg Thierry,
DiarraMehrpour Maryam
Publication year - 2002
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/ijc.10120
Subject(s) - metastasis , cancer research , trypsin , microbiology and biotechnology , green fluorescent protein , cell culture , chemistry , alpha (finance) , biology , cancer , medicine , gene , biochemistry , genetics , enzyme , construct validity , nursing , patient satisfaction
The inter‐α trypsin inhibitor (ITI) family is a group of proteins built up from different combinations of 1 light chain (ITI‐L) and 3 highly homologous heavy chains (ITI‐H1, ‐H2 and ‐H3). To investigate a potential role of the ITI family chains in cancer and metastasis spreading, we engineered human H460M cell lines expressing both the green fluorescent protein (GFP) and one of these chains. These clones were subcutaneously injected in athymic nude mice, and lung metastasis number and primary tumor weight were determined after 28 days. Expression of the ITI‐L chain considerably decreased tumor weight and fluorescent lung metastasis number. ITI‐H1 and ITI‐H3 chain expression induced a significant decrease of metastasis number, whereas no decrease of tumor weight could be detected. In vitro , ITI‐L expression significantly decreased chemotaxis and ITI‐H1 and ITI‐H3 expression increased cell attachment. These results argue for the antitumoral or antimetastatic properties of ITI‐L, ‐H1 and ‐H3 chains. © 2001 Wiley‐Liss, Inc.