Open AccessThe frequency of CD4+ and CD8+ circulating T stem cell memory in type 1 diabetes
Author(s) -
Fazeli Pooriya,
Talepoor Atefe Ghamar,
Faghih Zahra,
Gholijani Nasser,
Ataollahi Mohammad Reza,
AliHassanzadeh Mohammad,
Moravej Hossein,
Kalantar Kurosh
Publication year - 2022
Publication title -
immunity, inflammation and disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.918
H-Index - 18
ISSN - 2050-4527
DOI - 10.1002/iid3.715
Subject(s) - cd8 , fas receptor , flow cytometry , c c chemokine receptor type 7 , immunology , medicine , immune system , apoptosis , biology , biochemistry , chemokine , chemokine receptor , programmed cell death
The frequencies and functions of T stem cell memory (TSCM) subsets vary in autoimmune diseases. We evaluated the frequencies of CD4 + and CD8 + TSCM subsets as well as their PD‐1 expression levels in patients with T1D. Methods Blood samples were collected from new case (NC) ( n = 15), and long‐term (LT) ( n = 15) groups and healthy controls ( n = 15). Five subsets of T cells including TCM(CD4 + /CD8 + CCR7 + CD45RO + CD95 + ), TCM hi (CD4 + /CD8 + CCR7 + CD45RO hi CD95 + ), TEM(CD4 + /CD8 + CCR7 − CD45RO + CD95 + ), TSCM(CD4 + /CD8 + CCR7 + CD45RO − CD95 + ), and T naive (CD4 + /CD8 + CCR7 + CD45RO − CD95 − ) were detected by flow‐cytometry. Results The frequency of CD4 + TSCM was higher in NC patients than LT patients and controls ( p < .0001 and p = .0086, respectively). A higher percentage of the CD8 + T naive cells was shown in NC patients as compared with LT and healthy individuals ( p = .0003 and p = .0002, respectively). An increased level of PD‐1 expression was observed on the CD4 + TCM and TCM hi cells in LT patients as compared with healthy controls ( p = .0037 and p = .0145, respectively). Also, the higher PD‐1 expression was observed on the CD8 + TCM and TCM hi in NC and LT patients as compared with controls ( p = .0068 and p < .0001; p = .0012 and p = .0012, respectively). Conclusion Considering TSCMs' capacities to generate all memory and effector T cells, our results may suggest a potential association between the increased frequencies of TSCMs and T1D progression.