Open AccessThe role of inflammatory miRNA–mRNA interactions in PBMCs of colorectal cancer and obesity patients
Author(s) -
Gholami Morteza,
Zoughi Marziyeh,
Larijani Bagher,
Abdollahzadeh Rasoul,
Taslimi Reza,
Rahmani Zeinab,
Kazemeini Alireza,
Behboo Roobic,
Razi Farideh,
Bastami Milad,
HasaniRanjbar Shirin,
Amoli Mahsa M.
Publication year - 2022
Publication title -
immunity, inflammation and disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.918
H-Index - 18
ISSN - 2050-4527
DOI - 10.1002/iid3.702
Subject(s) - microrna , colorectal cancer , peripheral blood mononuclear cell , body mass index , medicine , gene expression , inflammation , oncology , cancer , transcriptome , obesity , bioinformatics , gene , immunology , biology , genetics , in vitro
Inflammation is a critical hallmark in obesity and colorectal cancer (CRC). This study aimed to investigate effective microRNA (miRNA)–messenger RNA (mRNA) interactions on inflammatory networks involved in obesity and CRC. Methods The literature searches were applied to identify genes expression reported on peripheral blood mononuclear cells (PBMCs) and/or blood of CRC subjects and to find inflammatory miRNA in blood samples. Furthermore, bioinformatics analysis was utilized to find inflammatory miRNA:mRNA interactions of the genes. Finally, a case‐control study was set to investigate the expression of LAMC1 and GNB3 genes besides miR‐10b, miR‐506‐3p, miR‐150‐5p, and miR‐124‐3p in CRC and control subjects. Results The expression of LAMC1 gene in healthy control groups was associated with body mass index (BMI) ( p < .05). The level of miR‐10b ( p < .001), miR‐506 ( p < .001), and miR‐124 ( p < . 001) were significantly increased in PBMCs of CRC patients, while they were not associated with BMI. The level of miR‐150 was associated with BMI in healthy subjects ( p < .05). Conclusions The changes in the level of miR‐506 and miR‐124 in CRC patients may be associated with the regulatory role of these miRNAs on LAMC1 expression. The LAMC1 may be related to BMI, however, more observational studies on other populations are needed.