Open AccessDifferences in immune responses between CMV‐seronegative and ‐seropositive patients with myocardial ischemia and reperfusion
Author(s) -
Shmeleva Evgeniya V.,
Boag Stephen E.,
Murali Santosh,
Bennaceur Karim,
Das Rajiv,
Egred Mohaned,
Purcell Ian,
Edwards Richard,
Todryk Stephen,
Spyridopoulos Ioakim
Publication year - 2015
Publication title -
immunity, inflammation and disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.918
H-Index - 18
ISSN - 2050-4527
DOI - 10.1002/iid3.49
Subject(s) - medicine , immune system , immunology , myocardial infarction , antibody
CMV infection is responsible for acceleration of immune senescence and linked to systemic pathologies, including cardiovascular diseases. In this study, we investigated differences in the immune response between CMV‐seropositive and seronegative patients undergoing primary percutaneous coronary intervention (PPCI) for acute myocardial infarction (MI). Peripheral blood samples were taken at six different time points: pre‐, 15, 30, 90 min, 24 h after PPCI and at 3 months after MI. Absolute counts of lymphocyte subpopulations, immune response to specific and nonspecific stimulation, serum cytokines and levels of CMV‐IgG, cardiolipin‐IgG, and anti‐endothelial cell antibodies were assessed. CMV‐seropositive patients with MI showed a twofold higher IFN‐γ production to PHA‐stimulation, up to 2.5‐fold higher levels of IP‐10 in serum and up to 30% lower serum levels of IL‐16 compared to CMV‐seronegative individuals. CMV‐seropositive patients could be divided into two subgroups with high (IL‐10Hi) and low (IL‐10Lo) IL‐10 serum levels during the acute stage of MI. The IL‐10Hi CMV‐seropositive subgroup showed an increased exit of late‐differentiated T lymphocytes, NK and NKT‐like cells from the circulation, which may potentially enhance cytotoxic damage in the ischemic myocardium. Finally, we did not observe an acceleration of autoimmunity by MI in CMV‐seropositive individuals. The immune response during acute MI showed characteristic differences between CMV seronegative and seropositive patients, with a stronger pro‐inflammatory response in seropositive patients. The effects of IP‐10, IL‐16, and IL‐10 on characteristics of acute immune responses and formation of different immune profiles in CMV‐seropositive individuals require further investigation.