Open AccessDetection of antigens and anti‐ Toxocara canis antibodies in children with different asthma severities
Author(s) -
Sandra Guadalupe BautistaGarcía,
Mario Noé MartínezGordillo,
Gustavo Esteban PeraltaAbarca,
Norma Yvett GonzálezBobadilla,
Karina ClavijoSánchez,
Alma Leticia ChávezZea,
Alan Eduardo HernándezSaavedra,
José Guadalupe HuertaLópez,
Álvaro PedrozaMeléndez,
Alejandro Gabriel GonzálezGaray,
Martha PonceMacotela
Publication year - 2021
Publication title -
immunity, inflammation and disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.918
H-Index - 18
ISSN - 2050-4527
DOI - 10.1002/iid3.403
Subject(s) - toxocara canis , canis , seroprevalence , asthma , immunology , immunoglobulin e , antigen , medicine , toxocariasis , antibody , ascaris , biology , helminths , serology , ecology
Toxocara canis can produce or exacerbate asthma, and the detection of anti‐ T. canis immunoglobulin G (IgG) does not discriminate between recent infection or active larva migrans . In this study, we searched for T. canis third‐stage larval antigens (L 3 TES) and anti‐ T. canis antibodies in children with different severities of asthma, controlled or uncontrolled. Methods A total of 145 patients with asthma who were previously diagnosed using the Global Initiative for Asthma guidelines were included. The asthma control was evaluated with the Asthma Control Questionnaire (ACQ). Enzyme‐linked immunosorbent assay was performed for the detection of L 3 TES; IgG was detected using sera preadsorbed with Ascaris antigens (native kit), and a commercial kit (IgG) was used as the gold standard. Results L 3 TES was found in 2 patients (1.37%). One had L 3 TES and anti‐ T. canis IgG, suggesting active larva migrans . In the other patient, only L 3 TES was detected, likely because an infection had begun. The seroprevalence with the commercial kit and native kit was 6.2% and 17.93%, respectively. There was no significant association among asthma severity, ACQ and T. canis seroprevalence ( p > .05). Conclusion It is possible to detect L 3 TES in patients with asthma. Two complementary techniques that can determine the infection status with T. canis and rule out cross‐reactions involve the detection of L 3 TES and IgG using sera preadsorbed with Ascaris antigen. There was no significant association among asthma severity, ACQ and T. canis seroprevalence.