Open AccessMyeloid suppressor cells require membrane TNFR2 expression for suppressive activity
Author(s) -
Polz Johannes,
Remke Annika,
Weber Sabine,
Schmidt Dominic,
WeberSteffens Dorothea,
PietrygaKrieger Anne,
Müller Nils,
Ritter Uwe,
Mostböck Sven,
Männel Daniela N.
Publication year - 2014
Publication title -
immunity, inflammation and disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.918
H-Index - 18
ISSN - 2050-4527
DOI - 10.1002/iid3.19
Subject(s) - myeloid derived suppressor cell , bone marrow , cd11c , myeloid , adoptive cell transfer , microbiology and biotechnology , chemistry , immunology , cancer research , biology , phenotype , t cell , suppressor , immune system , biochemistry , gene
TNF and TNF receptor type 2 (TNFR2) have been shown to be important for generation of myeloid‐derived suppressor cells (MDSC). In order to analyze whether and how TNFR2 passes the effect of TNF on, myeloid cells from TNFR2‐deficient mice were compared to respective cells from wild‐type mice. Primary TNFR2‐deficient myeloid cells showed reduced production of NO and IL‐6 which was attributable to CD11b + CD11c − Ly6C + Ly6G − immature monocytic MDSC. TNFR2‐deficient MDSC isolated from bone marrow were less suppressive for T cell proliferation compared to WT‐derived MDSC. These differences on myeloid cells between the two mouse lines were still observed after co‐culture of bone marrow cells from the two mouse lines together during myeloid cell differentiation, which demonstrated that the impaired functional capacity of TNFR2‐deficient cells was independent of soluble factors but required membrane expression of TNFR2. Similarly, adoptive transfer of TNFR2‐deficient bone marrow cells into wild‐type hosts did not rescue the TNFR2‐specific phenotype of bone marrow‐derived myeloid cells. Therefore, membrane TNFR2 expression determines generation and function of monocytic MDSC.