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Two novel biomarkers in developing wood frogs
Author(s) -
Gupta Niti,
Hitchings Amanda,
Weber Lynn,
Wickstrom Mark
Publication year - 2008
Publication title -
integrated environmental assessment and management
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.665
H-Index - 57
eISSN - 1551-3793
pISSN - 1551-3777
DOI - 10.1002/ieam.5630040118
Subject(s) - triglyceride , hormone , thyroid , homogenization (climate) , extraction (chemistry) , thyroid hormones , biology , chromatography , chemistry , medicine , endocrinology , ecology , cholesterol , biodiversity
Two novel biomarkers (whole-body thyroid hormone and triglyceride concentrations) were developed for use in wood frog tadpoles. The development of these biomarkers was based on previous work done in fish and lizards and incorporated triglyceride and thyroid hormone extraction into 1 homogenization step with 95% ethanol as the solvent. This method is useful for reducing sample-to-sample variability because the same sample can be used for each extraction and assay.