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Intra‐Accumbens trh prolongs maintenance of tolerance to hypothermic effect of ethanol in rats
Author(s) -
Pucilowski O.,
Trzaskowska E.,
Kostowski W.
Publication year - 1988
Publication title -
human psychopharmacology: clinical and experimental
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.461
H-Index - 78
eISSN - 1099-1077
pISSN - 0885-6222
DOI - 10.1002/hup.470030111
Subject(s) - nucleus accumbens , saline , ethanol , medicine , hypothermia , endocrinology , chemistry , anesthesia , central nervous system , biochemistry
Tolerance to the hypothermic effect of ethanol (Et–OH) developed in male Wistar rats treated daily with 5 g/kg of 20 per cent ET–OH v/v i.g. for 6 days. On Day 6 Et–OH treatment was discontinued and the animals were divided into two groups injected into the nucleus accumbens septi (NAS), either with pGlu‐His‐Pro‐NH 2 (TRH) or saline. The peptide was injected via permanently implanted cannulae in doses of 10 or 30 m̈g in 0.5 m̈l on each side once daily. The effect of such treatment on the maintenance of Et–OH tolerance was assessed by measuring the temperature decrement in response to a challenge dose of Et–OH (5 g/kg i.p.) given on Day 10. It was found that in saline‐treated rats the initial tolerance declined. TRH‐injected groups exhibited constantly low response to hypothermic action of Et–OH. In a control experiment TRH was shown to have no effect on the body temperature after intra‐accumbens application in Et–OH‐naive rats.