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Effect of atypical antipsychotics on antioxidant enzyme activities in human erythrocytes ( in vitro study)
Author(s) -
Miljević Čedo,
NikolićKokić Aleksandra,
Nikolić Milan,
Niketić Vesna,
Spasić Mihajlo B.,
LečićToševski Dušica,
Blagojević Duško
Publication year - 2013
Publication title -
human psychopharmacology: clinical and experimental
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.461
H-Index - 78
eISSN - 1099-1077
pISSN - 0885-6222
DOI - 10.1002/hup.2272
Subject(s) - sertindole , quetiapine , aripiprazole , chemistry , pharmacology , glutathione peroxidase , atypical antipsychotic , olanzapine , glutathione reductase , amisulpride , antioxidant , superoxide dismutase , biochemistry , antipsychotic , medicine , schizophrenia (object oriented programming) , psychiatry
Objective This study was set out to examine the impact of atypical antipsychotic drugs: aripiprazole, clozapine, ziprasidone, olanzapine, quetiapine, sertindole and amisulpride on the activity of antioxidant defence enzymes in human erythrocytes in vitro . Methods Cu,Zn‐superoxide dismutase (SOD1), catalase (CAT), selenium‐dependent glutathione peroxidase and glutathione reductase activities were determined after drugs incubation with blood of 15 apparently healthy non‐smoking male volunteers (ages 23–39) for 1 h at 37 °C. Results A statistically significant increase in SOD1 activity was found in samples incubated with aripiprazole ( p < 0.01) and quetiapine ( p < 0.05) compared with incubated control. SOD1 activity profile following native polyacrylamide gel electrophoresis indicates that aripiprazole and quetiapine protect enzyme activity from inhibition with hydrogen peroxide. Our results showed that sertindole decreases activity of CAT comparing with control non‐treated erythrocytes. Moreover, in sertindole treated erythrocytes, negative correlation between SOD1 and glutathione peroxidase activities was found. Increased amount of hydrogen peroxide in such situation may leave erythrocytes and transform their role in circulation from anti‐oxidative to pro‐oxidative. Conclusions Our results indicate that mechanism through sertindole could express its in vivo toxic effects and point toward possible (neuro)protective effects of aripiprazole and quetiapine. Copyright © 2012 John Wiley & Sons, Ltd.