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Analysis of the PTCH coding region in human rhabdomyosarcoma
Author(s) -
CalzadaWack Julia,
Schnitzbauer Udo,
Walch Axel,
Wurster KarlHeinz,
Kappler Roland,
Nathrath Michaela,
Hahn Heidi
Publication year - 2002
Publication title -
human mutation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 162
eISSN - 1098-1004
pISSN - 1059-7794
DOI - 10.1002/humu.9056
Subject(s) - nevoid basal cell carcinoma syndrome , medulloblastoma , biology , rhabdomyosarcoma , missense mutation , exon , ptch1 , cancer research , nonsense mutation , patched , locus (genetics) , genetics , basal cell carcinoma , gene , pathology , mutation , sarcoma , basal cell , medicine , hedgehog signaling pathway
Inherited mutations of the human tumor suppressor gene Patched ( PTCH ) lead to an autosomal dominant disorder known as Nevoid Basal Cell Carcinoma Syndrome (NBCCS). The syndrome is characterized by a combination of developmental abnormalities and a predisposition to tumor formation. Tumors in patients with NBCCS include basal cell carcinoma, medulloblastoma, fibroma and rhabdomyosarcoma (RMS). RMS are also present in 15 % of mice haplodeficient for Ptch . To investigate whether mutations in PTCH are a general feature in rhabdomyosarcomagenesis we sequenced the protein‐coding region in sporadic human cases of these tumors. For this purpose we first determined the distribution and frequency of polymorphisms in 23 exons of PTCH in 48 healthy caucasians. Ten new polymorphisms were identified (IVS11 + 15‐17del AAA; IVS14 + 25T>C; 2485G>A; IVS15 + 9G>C; IVS17 + 21A>G; 3033T>C; 3149T>C; 3387T>C; 3617G>A; 4080C>T). Next, the PTCH coding region in 14 RMS was sequenced. Whereas one case with LOH at the PTCH locus was detected, none of the cases showed nonsense or missense mutations in the coding region of PTCH . These data do not support the existence of frequent mutations in the protein‐coding region of PTCH in RMS. © 2002 Wiley‐Liss, Inc.