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Benchmarking predictions of allostery in liver pyruvate kinase in CAGI4
Author(s) -
Xu Qifang,
Tang Qingling,
Katsonis Panagiotis,
Lichtarge Olivier,
Jones David,
Bovo Samuele,
Babbi Giulia,
Martelli Pier L.,
Casadio Rita,
Lee Gyu Rie,
Seok Chaok,
Fenton Aron W.,
Dunbrack Roland L.
Publication year - 2017
Publication title -
human mutation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 162
eISSN - 1098-1004
pISSN - 1059-7794
DOI - 10.1002/humu.23222
Subject(s) - allosteric regulation , biology , computational biology , pyruvate kinase , effector , bioinformatics , genetics , biochemistry , enzyme , glycolysis
The Critical Assessment of Genome Interpretation (CAGI) is a global community experiment to objectively assess computational methods for predicting phenotypic impacts of genomic variation. One of the 2015–2016 competitions focused on predicting the influence of mutations on the allosteric regulation of human liver pyruvate kinase. More than 30 different researchers accessed the challenge data. However, only four groups accepted the challenge. Features used for predictions ranged from evolutionary constraints, mutant site locations relative to active and effector binding sites, and computational docking outputs. Despite the range of expertise and strategies used by predictors, the best predictions were marginally greater than random for modified allostery resulting from mutations. In contrast, several groups successfully predicted which mutations severely reduced enzymatic activity. Nonetheless, poor predictions of allostery stands in stark contrast to the impression left by more than 700 PubMed entries identified using the identifiers “computational + allosteric.” This contrast highlights a specialized need for new computational tools and utilization of benchmarks that focus on allosteric regulation.