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Transgenic mice carrying the H258N mutation in the gene encoding the β‐subunit of phosphodiesterase‐6 (PDE6B) provide a model for human congenital stationary night blindness
Author(s) -
Tsang Stephen H.,
Woodruff Michael L.,
Jun Lin,
Mahajan Vinit,
Yamashita Clyde K.,
Pedersen Robert,
Lin ChyuanSheng,
Goff Stephen P.,
Rosenberg Thomas,
Larsen Michael,
Farber Debora B.,
Nusinowitz Steven
Publication year - 2007
Publication title -
human mutation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 162
eISSN - 1098-1004
pISSN - 1059-7794
DOI - 10.1002/humu.20425
Subject(s) - rhodopsin , retinitis pigmentosa , biology , retinal degeneration , transgene , phosphodiesterase , transducin , allele , erg , mutation , visual phototransduction , genetics , mutant , retinal , microbiology and biotechnology , gene , biochemistry , enzyme
Abstract Mutations in the β‐subunit of cGMP‐phosphodiesterase (PDE6β) can lead to either progressive retinal disease, such as human retinitis pigmentosa (RP), or stationary disease, such as congenital stationary night blindness (CSNB). Individuals with CSNB in the Rambusch pedigree were found to carry the H258N allele of PDE6B (MIM# 180072); a similar mutation was not found in RP patients. This report describes an individual carrying the H258N allele, who presented with generalized retinal dysfunction affecting the rod system and a locus of dysfunction at the rod‐bipolar interface. Also described are preclinical studies in which transgenic mice with the H258N allele were generated to study the pathophysiological mechanisms of CSNB. While Pde6b rd1 / Pde6b rd1 mice have severe photoreceptor degeneration, as in human RP, the H258N transgene rescued these cells. The cGMP‐PDE6 activity of dark‐adapted H258N mice showed an approximate three‐fold increase in the rate of retinal cGMP hydrolysis: from 130.1 nmol × min −1 × nmol −1 rhodopsin in wild‐type controls to 319.2 nmol × min −1 × nmol −1 rhodopsin in mutants, consistent with the hypothesis that inhibition of the PDE6β activity by the regulatory PDE6γ subunit is blocked by this mutation. In the albino (B6CBA × FVB) F2 hybrid background, electroretinograms (ERG) from H258N mice were similar to those obtained from affected Rambusch family members, as well as humans with the most common form of CSNB (X‐linked), demonstrating a selective loss of the b ‐wave with relatively normal a ‐waves. When the H258N allele was introduced into the DBA background, there was no evidence of selective reduction in b ‐wave amplitudes; rather a ‐ and b ‐wave amplitudes were both reduced. Thus, factors other than the PDE6B mutation itself could contribute to the variance of an electrophysiological response. Therefore, caution is advisable when interpreting physiological phenotypes associated with the same allele on different genetic backgrounds. Nevertheless, such animals should be of considerable value in further studies of the molecular pathology of CSNB. Hum Mutat 28(3), 243–254, 2007. Published 2006 Wiley‐Liss, Inc.