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Identification of rare and novel mutations in the CFTR genes of CF patients in Southern England
Author(s) -
Shackleton Sue,
Hull Jeremy,
Dear Simon,
Seller Anneke,
Thomson Anne,
Harris Ann
Publication year - 1994
Publication title -
human mutation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 162
eISSN - 1098-1004
pISSN - 1059-7794
DOI - 10.1002/humu.1380030209
Subject(s) - biology , identification (biology) , genetics , gene , mutation , computational biology , ecology
Abstract Cystic fibrosis patients referred to two genetics centres in southern England and not found to carry common CF‐associated mutations in one or both of their CFTR genes have been subjected to an extensive mutation search. The whole of the coding region of the CFTR gene, all intron–exon boundaries and 5′ and 3′ untranslated regions have been examined by a combination of single stranded conformational polymorphism analysis and chemical mismatch detection; 48 chromosomes with rare mutations have been identified, including 7 novel mutations, 182delT in exon 1, G27X in exon 2, Q151X in exon 4, Q220X in exon 6a, Q525X in exon 10, 3041delG in exon 16, and 4271delC in exon 23. © 1994 Wiley‐Liss, Inc.