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Mutation detection by denaturing gradient gel electrophoresis (DGGE)
Author(s) -
Fodde Riccardo,
Losekoot Monique
Publication year - 1994
Publication title -
human mutation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 162
eISSN - 1098-1004
pISSN - 1059-7794
DOI - 10.1002/humu.1380030202
Subject(s) - temperature gradient gel electrophoresis , biology , gel electrophoresis , dna , rnase p , microbiology and biotechnology , genetics , gene , polyacrylamide gel electrophoresis , computational biology , biochemistry , enzyme , 16s ribosomal rna , rna
The molecular analysis of genetic diseases relies on several technical approaches which allow genetic and physical mapping, characterization of the gene structure, expression studies, and identification of disease‐causing mutations. Denaturing gradient gel electrophoresis (DGGE) allows the rapid screening for single base changes in enzymatically amplified DNA. The technique is based on the migration of double‐stranded DNA molecules through polyacrylamide gels containing linearly increasing concentrations of a denaturing agent. In this review DGGE and the several modifications of the original protocol are presented. Moreover, its applications in human molecular genetics are summarized together with a preliminary comparison with other mutation detection technologies such as chemical cleavage, RNase protection, and single‐strand conformation polymorphism. © 1994 Wiley‐Liss, Inc.