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A method to isolate DNA from small archival tissue samples for p53 gene analysis
Author(s) -
Schubert Elizabeth L.,
Bischoff Farideh Z.,
Whitaker Laura L.,
Pleasants Lisa M.,
Hansen Marc F.
Publication year - 1993
Publication title -
human mutation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 162
eISSN - 1098-1004
pISSN - 1059-7794
DOI - 10.1002/humu.1380020211
Subject(s) - biology , li–fraumeni syndrome , germline mutation , germline , gene , mutation , dna , genetics , tumor suppressor gene , cancer research , dna extraction , microbiology and biotechnology , carcinogenesis , polymerase chain reaction
The tumor suppressor gene p53 is involved in predisposition to a variety of human cancers, including those from Li–Fraumeni cancer family syndrome patients. Studies of inheritance of p53 germline mutations require confirmation of the mutation in the tumors from family members. These studies as well as other retrospective studies of tumor specific mutations, are often hampered by a lack of available fresh or frozen tumor tissue samples for DNA extraction to confirm the suspected p53 mutation. Here we describe a simple technique for DNA isolation that permits mutational analysis of p53 from minimal amounts of paraffin‐embedded archival tissue samples. © 1993 Wiley‐Liss, Inc.