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RNase cleavage‐based methods for mutation/SNP detection, past and present
Author(s) -
Goldrick Marianna M.
Publication year - 2001
Publication title -
human mutation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 162
eISSN - 1098-1004
pISSN - 1059-7794
DOI - 10.1002/humu.1175
Subject(s) - cleave , rnase p , biology , rnase h , cleavage (geology) , genetics , ribonuclease , nucleic acid , rna , gene , dna , rnase mrp , microbiology and biotechnology , computational biology , paleontology , fracture (geology)
Mutation detection based on ribonuclease cleavage of basepair mismatches in single‐stranded RNA probes hybridized to DNA targets was first described over 15 years ago. The original methods relied on RNase A for mismatch cleavage; however, this enzyme fails to cleave many mismatches and has other drawbacks. More recently, a new method for RNase‐cleavage‐based mutation scanning has been developed, which takes advantage of the ability of RNase 1 and RNase T1 to cleave mismatches in duplex RNA targets, when these enzymes are used in conjunction with nucleic acid intercalating dyes. The method, called NIRCA, is relatively low‐cost in terms of materials and equipment required. It is being used to detect mutations and SNPs in a wide variety of genes involved in human genetic disease and cancer, as well as in disease‐related viral and bacterial genes. This review describes historical and recently developed RNase cleavage‐based methods for mutation/SNP scanning. Hum Mutat 18:190–204, 2001. © 2001 Wiley‐Liss, Inc.