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Making ends meet in genetic analysis using padlock probes
Author(s) -
Nilsson Mats,
Banér Johan,
MendelHartvig Maritha,
Dahl Fredrik,
Antson DanOscar,
Gullberg Mats,
Landegren Ulf
Publication year - 2002
Publication title -
human mutation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.981
H-Index - 162
eISSN - 1098-1004
pISSN - 1059-7794
DOI - 10.1002/humu.10073
Subject(s) - biology , computational biology , rolling circle replication , genetics , replication (statistics) , sequence (biology) , rna , dna , dna sequencing , dna replication , gene , virology
Abstract Padlock probes are molecular tools that combine highly specific target sequence recognition with the potential for multiplexed analysis of large sets of target DNA or RNA sequences. In this brief review, we exemplify the ability of these probes to distinguish single‐nucleotide target sequence variants. We further discuss means to detect the location of target sequences in situ, and to amplify reacted padlock probes via rolling‐circle replication, as well as to sort reaction products on tag‐arrays. We argue that the probes have the potential to render high‐throughput genetic analyses precise and affordable. Hum Mutat 19:410–415, 2002. © 2002 Wiley‐Liss, Inc.