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Cell kinetic analysis of non‐Hodgkin's lymphomas using in vivo iododeoxyuridine incorporation and flow cytometry
Author(s) -
Erlanson Martin,
Lindh Jack,
Zackrisson Björn,
Landberg Göran,
Roos Göran
Publication year - 1995
Publication title -
hematological oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.918
H-Index - 44
eISSN - 1099-1069
pISSN - 0278-0232
DOI - 10.1002/hon.2900130405
Subject(s) - doubling time , flow cytometry , propidium iodide , biopsy , in vivo , medicine , lymphoma , non hodgkin's lymphoma , pathology , radioimmunotherapy , staining , antibody , biology , cell , immunology , apoptosis , monoclonal antibody , biochemistry , genetics , microbiology and biotechnology , programmed cell death
The aim of this study was to analyse dynamic cell proliferation parameters in non‐Hodgkin's lymphomas. Sixty‐one patients with newly diagnosed or with recurrent disease were given iododeoxyuridine (IdUrd) intravenously near 4 h prior to tumour biopsy. After staining with an IdUrd reactive antibody and propidium iodide, S‐phase fraction (SPF), labelling index (LI), S‐phase duration time ( T s) and potential tumour doubling time ( T pot) were determined by flow cytometry. Thirty‐eight samples, 15 low grade (LGM) and 23 high grade (HGM) malignant lymphomas, were possible to evaluate. Twenty‐three cases were excluded due to aneuploidy, insufficient amount of material or technical problems. T pot values varied between 0.8–32.9 days (mean 7.0 days). HGM lymphomas had shorter mean T pot times than LGM lymphomas (4.8 versus 10.4 days, p =0.05). For T s the range was 4.2–20.1 h (mean 9.1 h), and a difference between the two histological groups was demonstrated with a longer mean T s for HGM compared with LGM cases (10.0 versus 7.8 h, p =0.04). T pot showed a negative correlation with SPF ( P =0.003), and T s demonstrated a positive correlation to SPF ( p =0.02). The clinical significance of the dynamic cell proliferation parameters studied remains to be clarified, but the interelationships between T s/SPF and T s/morphologic subtype might be factors of interest for future prognostic studies in malignant lymphomas.