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Interleukin‐10 production by a B‐cell line derived from human post‐transplant lymphoproliferative disease
Author(s) -
Randhawa Parmjeet,
Nalesnik Michael,
Demetris Jake,
Zeevi Adriana
Publication year - 1995
Publication title -
hematological oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.918
H-Index - 44
eISSN - 1099-1069
pISSN - 0278-0232
DOI - 10.1002/hon.2900130103
Subject(s) - lymphoproliferative disease , immunology , b cell , medicine , disease , lymphoma , antibody
Interleukin‐10 (IL‐10) is a cytokine known to regulate growth and differentiation in activated human B cells. We studied IL‐10 production in a B‐cell line derived from Epstein‐Barr virus associated post‐transplant lymphoproliferative disease (PTLD). Reverse transcriptase polymerase chain reaction (RTPCR) demonstrated IL‐10 mRNA within the cells. Transcripts of the virally encoded homologue BCRF‐1 were not detected. ELISA assays demonstrated translation of IL‐10 message into the corresponding cytokine, and its subsequent secretion into the culture medium. The rate of 3 H‐thymidine incorporation by the PTLD cells was not affected by immunologic neutralization of the secreted cytokine, or, by addition of exogenous recombinant IL‐10 to the culture medium. Thus, IL‐10 does not have an autocrine growth regulatory role in this PTLD line.