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Transfection of the bcr/abl oncogene into factor‐dependent cells by electroporation: acquisition of autonomous proliferation
Author(s) -
Takahashi Masuhiro,
Furukawa Tatsuo,
Tanaka Izumi,
Ohsawa Yukiko,
Nikkuni Kohji,
Aoki Azusa,
Goto Takao,
Hashimoto Takao,
Kishi Kenji,
Koike Tadashi,
Moriyama Yoshiaki,
Shibata Akira
Publication year - 1994
Publication title -
hematological oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.918
H-Index - 44
eISSN - 1099-1069
pISSN - 0278-0232
DOI - 10.1002/hon.2900120202
Subject(s) - transfection , electroporation , oncogene , clone (java method) , biology , cancer research , cell culture , microbiology and biotechnology , haematopoiesis , cell , stem cell , dna , cell cycle , gene , genetics
In order to clarify the function of P210 bcr/abl oncogene in leukemogenesis, IL‐3 dependent murine hematopoietic cell line, FDC‐P2, was transfected with the plasmid containing cDNA of P210 bcr/abl oncogene (pGD'210) or murine IL‐3 (pcDmIL3) by electroporation. Four out of five pGD'210 transfected clones as well as FDC‐P2 transfected with pcDmIL3, acquired autonomous proliferation (i.e. lost the requirement for IL‐3 supplementation). The expression of bcr/abl oncogene was weak in one clone, which remained dependent on IL‐3. Unlike pcDmIL3 transfectants, which secrete IL‐3 into the supernatant, IL‐3 was not demonstrated in the culture supernatant of pGD'210 transfected FDC‐P2. These findings suggest that P210 bcr/abl oncogene is directly associated with autonomous proliferation, which is the first process of leukemogenesis.