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Protein phosphatase 2Aα involvement in granulocytic differentiation of HL‐60 cells
Author(s) -
Sasaki Kazunori,
Ikeda Kazuma,
Nagai Masami,
Shima Hiroshi,
Nagao Minako,
Takahara Jiro,
Irino Shozo
Publication year - 1993
Publication title -
hematological oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.918
H-Index - 44
eISSN - 1099-1069
pISSN - 0278-0232
DOI - 10.1002/hon.2900110102
Subject(s) - alpha (finance) , phosphatase , mitosis , dimethyl sulfoxide , haematopoiesis , cellular differentiation , microbiology and biotechnology , cell growth , biology , chemistry , biochemistry , phosphorylation , gene , stem cell , medicine , construct validity , nursing , organic chemistry , patient satisfaction
Protein phosphatase 2Aα (PP‐2Aα) is one of the catalytic subunits of PP‐2A, which is composed of catalytic and regulatory subunits. In fission yeasts, PP‐2A mutants cause mitotic defects and strikingly different cell cycle phenotypes. In rat hepatocellular carcinomas, mRNA expression of PP‐2Aα was increased compared to that of normal hepatocytes. From these studies, PP‐2Aα has been thought to be involved in proliferation, and we expected that PP‐2Aα would decrease in HL‐60 cells on dimethyl sulfoxide(DMSO)‐induced granulocytic differentiation with decreased growth rate. In this study, we show that PP‐2Aα expression increases in HL‐60 cells on DMSO‐induced granulocytic differentiation, while PP‐1γ , which is one of catalytic subunits of protein phosphatase 1, expression did not change significantly. These results suggest that PP‐2Aα may be involved in regulation of differentiation of hematopoietic cells.