Premium
In vitro synergistic activity of PDN‐IFNα and NM + IFNα combinations on fresh bone‐marrow samples from multiple myeloma patients
Author(s) -
Lepri Enrica,
Barzi Anna,
Menconi Emma,
Portuesi Maria Giovanna,
Liberati Marina
Publication year - 1991
Publication title -
hematological oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.918
H-Index - 44
eISSN - 1099-1069
pISSN - 0278-0232
DOI - 10.1002/hon.2900090203
Subject(s) - bone marrow , multiple myeloma , in vitro , medicine , cytotoxicity , cytotoxic t cell , cancer research , interferon , myeloid , population , immunology , microbiology and biotechnology , pathology , chemistry , biology , biochemistry , environmental health
The differential staining cytotoxicity (DiSC) assay was used to evaluate the in vitro sensitivity of tumour and normal bone‐marrow cells from 21 multiple myeloma (MM) patients to antitumour agents methylprednisolone (PDN), nitrogen mustard (NM) and recombinant interferon α‐2b (IFNα) tested singly and in the combinations PDN+IFNα and NM+IFNα. Both the PDN‐IFNα and NM‐IFNα associations were more efficacious than any agents used singly in reducing the percentage of myeloma cell survival. However, whereas NM, alone and in combination with IFNα, provoked a severe reduction in normal bone‐marrow population, PDN and PDN+IFNα induced an increase percentage survival of normal bone‐marrow cells. These findings indicate that, at least in vitro , the PDN‐IFNα combination exerts a great antitumour effect which is not associated with a relevant cytotoxic activity on normal myeloid cells.