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Triple combination of retinoic acid + low concentration of cytosine arabinoside + hexamethylene bisacetamide induces differentiation of human aml blasts in primary culture
Author(s) -
Hassan H. T.,
Rees J. K. H.
Publication year - 1989
Publication title -
hematological oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.918
H-Index - 44
eISSN - 1099-1069
pISSN - 0278-0232
DOI - 10.1002/hon.2900070606
Subject(s) - retinoic acid , cytarabine , cytosine , cytotoxic t cell , chemotherapy , leukemia , medicine , cancer research , cell culture , tretinoin , pharmacology , chemistry , immunology , in vitro , biology , biochemistry , dna , genetics
Differentiation induction therapy provides an alternative therapeutic approach for patients with acute myeloid leukemia (AML) who are either unsuitable for or unresponsive to conventional cytotoxic chemotherapy. The effect of a triple combination of retinoic acid (RA) + low concentration of cytosine arabinoside (Ara‐C) + hexamethylene bisacetamide (HMBA) on differentiation of blasts from 24 AML patients was studied. Non‐adherent mononuclear cells were seeded at a concentration of 5 × 10 5 cells per ml in 24‐well tissue culture plates containing RPMI 1640 culture medium with 20 per cent fetal calf serum and 10 per cent 5637‐conditioned medium and incubated with 10 −6 M retinoic acid, 10 −6 M cytosine arabinoside and or 2 mM hexamethylene bisacetamide for six days at 37 °C in a humidified incubator under 5 per cent CO 2. Morphological, cytochemical and functional differentiation into mature cells were induced in blasts from 22 out of the 24 AML patients following exposure to the triple combination of 10 −6 M RA + 10 −6 M Ara‐C+2 mM HMBA in primary culture. These effective results justify a clinical trial of such triple combination for AML patients who are either unsuitable for or unresponsive to conventional cytotoxic chemotherapy.