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Clonal antigen receptor gene rearrangements and epstein‐barr viral DNA in tissues of Hodgkin's disease
Author(s) -
Weiss Lawrence M.,
Warnke Roger A.,
Sklar Jeffrey
Publication year - 1988
Publication title -
hematological oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.918
H-Index - 44
eISSN - 1099-1069
pISSN - 0278-0232
DOI - 10.1002/hon.2900060306
Subject(s) - biology , southern blot , gene , reed–sternberg cell , gene rearrangement , genome , antibody , virology , immunoglobulin heavy chain , epstein–barr virus , population , dna , immunoglobulin light chain , lymphoma , virus , restriction fragment length polymorphism , microbiology and biotechnology , polymerase chain reaction , genetics , immunology , medicine , hodgkin lymphoma , environmental health
In an initial survey of 16 cases of Hodgkin's disease, tissues from one case of nodular sclerosing Hodgkin's disease, a recurrence with numerous Reed‐Sternberg cells, demonstrated faint heavy‐ and light‐chain immunoglobulin gene rearrangements. Analysis of seven additional similar cases with extremely numerous Reed‐Sternberg cells revealed that six of these seven cases contained clonally rearranged heavy‐ or light‐chain genes. In addition, the original biopsy specimen from the index case (obtained two years prior to the recurrence) had the same pattern of rearrangements of the immunoglobulin genes. In contrast, a germline configuration was observed for the β T cell receptor gene in all cases. These cases of Hodgkin's disease were also investigated for the presence of Epstein‐Barr viral (EBV) genomes by Southern and slot‐blot DNA hybridization analysis. Tissues from four of the 21 case studied showed evidence of EBV DNA sequences. Uninvolved lymphoid tissue from two of the positive cases failed to demonstrate viral DNA. To assess clonality of the cells containing the EBV genomes, the tissues positive for EBV DNA were also hybridized with a restriction fragment probe for the terminal sequences of the EBV genome. By this analysis three of the four cases demonstrated a clonal population of EBV‐infected cells.