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In vitro production of granulocyte–macrophage colony‐stimulating activity by murine bone marrow and spleen following vinblastine administration IN VIVO
Author(s) -
Williams Douglas E.,
Chervinsky David S.,
Orsini Frank R.,
Tebbi Cameron K.,
Fitzpatrick John E.
Publication year - 1987
Publication title -
hematological oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.918
H-Index - 44
eISSN - 1099-1069
pISSN - 0278-0232
DOI - 10.1002/hon.2900050105
Subject(s) - bone marrow , vinblastine , in vivo , spleen , granulocyte , medicine , in vitro , macrophage , haematopoiesis , pharmacology , immunology , chemistry , biology , chemotherapy , stem cell , biochemistry , genetics , microbiology and biotechnology
The production of granulocyte–macrophage colony‐stimulating activity (CSA) by isolated murine femur shafts, non‐dispersed bone marrow and spleens was assessed following administration of Vinblastine (VLB). These organs were removed from 2 h to 10 days post‐VLB and allowed to condition endotoxin‐free medium for 48 h. CSA activity was assessed using a soft agar cloning system. The data demonstrate that CSA elaboration was maximal 24 h post‐VLB, corresponding to the nadir of bone marrow GM‐CFC, and 6 h after splenic reached a minimum. No relationship between peripheral blood granulocyte count or serum endotoxin levels were observed.