Premium
Cytotoxic and DNA‐inhibitory effects of iron chelators on human leukaemic cell lines
Author(s) -
Kontoghiorghes George J.,
Piga Andrea,
Hoffbrand A. Victor
Publication year - 1986
Publication title -
hematological oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.918
H-Index - 44
eISSN - 1099-1069
pISSN - 0278-0232
DOI - 10.1002/hon.2900040303
Subject(s) - cytotoxic t cell , hl60 , tropolone , cytotoxicity , chemistry , dna synthesis , biochemistry , dna , thymidine , inhibitory postsynaptic potential , cell culture , incubation , k562 cells , chelation , microbiology and biotechnology , cell , in vitro , biology , organic chemistry , endocrinology , genetics
Cytotoxic and DNA synthesis inhibitory effects of several iron chelators with different physicochemical properties have been tested in four myeloid leukaemic cell lines (U937, K562, ML 2 and HL60). The small lipophilic chelators 8‐hydroxyquinoline, tropolone an5d omadine at 2 × 10 − M, caused substantial inhibition of labelled leucine and thymidine uptake into cells and also cell death following 4‐h incubation. These effects were approximately 10‐fold increased when the drugs were pre‐incubated with equimolar amounts of iron. Iron alone and hydrophilic chelators e.g. desferrioxamine had insignificant cytotoxic and DNA synthesis inhibitory effects under the same conditions.