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Prognostic significance of DNA‐analysis by flow cytometry in non‐Hodgkin's lymphoma
Author(s) -
Roos Göran,
Dige Ulrik,
Lenner Per,
Lindh Jack,
Johansson Hemming
Publication year - 1985
Publication title -
hematological oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.918
H-Index - 44
eISSN - 1099-1069
pISSN - 0278-0232
DOI - 10.1002/hon.2900030403
Subject(s) - malignancy , lymphoma , flow cytometry , ploidy , lymph node , medicine , pathology , significant difference , biology , oncology , gastroenterology , immunology , genetics , gene
Abstract DNA analysis by flow cytometry was performed on lymph node cells obtained from 65 untreated patients with non‐Hodgkin's lymphoma. According to the Kiel classification 41 cases were of low grade malignancy and 24 cases belonged to the high grade malignancy group. 47 out of 61 evaluable cases were diploid/near‐diploid, 12 were aneuploid and 2 cases showed polyploidy. No difference in survival was found between diploid/near‐diploid and aneuploid cases. The percentage of S‐phase cells was found to be a valuable prognostic parameter. Using a cut point at 4 per cent S‐phase cells between low and high S‐phase lymphomas a highly significant difference in survival was demonstrated ( p = 0.0004). The actuarial survival up to 3 years was about 77 per cent for lymphomas with <4 per cent S‐phase cells to be compared with 20 per cent for patients with high S‐phase (≥ 4 per cent) lymphomas. Subdivision according to the percentage of S‐phase cells seemed to be a better prognostic predictor than morphologic subclassification.