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Relationship between myeloblasts and cluster‐forming cells in acute non‐lymphocytic leukemia
Author(s) -
Gustavsson Anita,
Olofsson Tor,
Olsson Inge
Publication year - 1984
Publication title -
hematological oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.918
H-Index - 44
eISSN - 1099-1069
pISSN - 0278-0232
DOI - 10.1002/hon.2900020210
Subject(s) - cluster (spacecraft) , biology , leukemia , cancer research , immunology , computer science , programming language
The relationship between myeloblast‐like cells and cluster‐forming cells detected by growth in agar has been studied in 22 untreated patients with acute non‐lymphocytic leukemia by means of comparing data for 3 H‐TdR labelling of myeloblast‐like cells and 3 H‐TdR suiciding of cluster‐forming cells. The fraction of myeloblast‐like cells in S‐phase was significantly smaller (13·1 ± 8·7 per cent) than the fraction of cluster‐forming cells in S‐phase (35·5 ± 15·3 per cent). In 2/22 patients approximately half of the myeloblast‐like cells in DNA‐synthesis were calculated to produce clusters in agar which may suggest similarity for cell cycle active myeloblast‐like cells and cluster‐forming cells. The other patients showed decreased plating efficiency for myeloblast‐like cells in S‐phase indicating non‐identity or modification of the growth pattern due to environmental factors and variations in death rate during culture. Sequential studies in three patients indicated a changing relationship between myeloblast‐like cells and cluster‐forming cells suggesting non‐identity or differences in growth regulation during the course of the disease.