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Tartrate‐resistant acid phosphatase as a differentiation marker for the human mononuclear phagocyte system
Author(s) -
Radzun H. J.,
Kreipe H.,
Parwaresch M. R.
Publication year - 1983
Publication title -
hematological oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.918
H-Index - 44
eISSN - 1099-1069
pISSN - 0278-0232
DOI - 10.1002/hon.2900010404
Subject(s) - mononuclear phagocyte system , acid phosphatase , phagocyte , lymphokine , peripheral blood mononuclear cell , stimulation , immune system , tartrate resistant acid phosphatase , biology , immunology , chemistry , biochemistry , enzyme , endocrinology , in vitro
Human blood monocytes (BM) were stimulated with various immune modulators in short‐term cultures. Tartrate‐resistant acid phosphatase (TAcP) activity was demonstrated with an enzyme cyto‐chemical method. Other members of the mononuclear phagocyte system (MPS), such as peritoneal (PM) and alveolar macrophages (AM), were also tested. Unstimulated BM and physiologic functional forms of macrophages, with the exception of AM, were invariably TAcP negative. On appropriate stimulation, particularly with media containing lymphokines, cultured BM became TAcP positive. The results suggest that TAcP is an inducible differentiation marker that indicates transformation of monocytes into cells belonging to a distinct subset of the MPS.