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Phenotypic and cytogenetic characteristics of a new epstein‐barr virus negative cell line (SKW 4) derived from A B‐Cell lymphoma
Author(s) -
Nilsson Kenneth,
Sundström Christer,
Klareskog Lars,
Ralph Peter,
Zech Lore
Publication year - 1983
Publication title -
hematological oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.918
H-Index - 44
eISSN - 1099-1069
pISSN - 0278-0232
DOI - 10.1002/hon.2900010402
Subject(s) - lymphoma , biology , epstein–barr virus , virus , antigen , phenotype , chromosomal translocation , microbiology and biotechnology , virology , cancer research , immunology , genetics , gene
A new Epstein‐Barr virus nuclear antigen (EBNA) negative cell line SKW 4 has been established in vitro from a patient with diffuse histiocytic lymphoma. The SKW 4 seems to be an authentic human tumour cell line as evidenced by its EBV negativity, monoclonality and aneuploidy tested during early in vitro passage. The cell line expresses surface μ and k k ‐chains, HLA‐DR antigen, C3 and Fc receptors and B‐cell lineage antigens. The karyotypic analyses demonstrated many numerical and structural aberrations. No Burkitt lymphoma associated translocations (t8; 14 , t2; 8 , t;22) were detected, but most of the markers found are those commonly associated with various types of human cancer. The SKW 4 thus represents the most common type of ‘histiocytic lymphoma’, that with a B‐lymphoid cell phenotype, but is unique among HL derived lymphoma lines in its strong expression of a Helix pomatia A agglutinin binding surface glycoprotein of an apparent molecular weight of 75 000 daltons.