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Rapid detection of t(15;17)(q24;q21) in acute promyelocytic leukaemia by microwave‐assisted fluorescence in situ hybridization
Author(s) -
Soriani Silvia,
Mura Cinzia,
Panico Anna Rita,
Scarpa Anna Maria,
Recchimuzzo Patrizia,
Dadati Raffaella,
Farioli Renata,
De Canal Gabriella,
Mura Maria Angela,
Cesana Clara
Publication year - 2017
Publication title -
hematological oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.918
H-Index - 44
eISSN - 1099-1069
pISSN - 0278-0232
DOI - 10.1002/hon.2194
Subject(s) - in situ , fluorescence in situ hybridization , microwave , fluorescence , medicine , chemistry , physics , biochemistry , optics , organic chemistry , quantum mechanics , chromosome , gene
Acute promyelocytic leukaemia (APL) is a hematologic malignancy characterized by the rearrangement of the PML and RARα genes, mostly due to a reciprocal chromosomal translocation t(15;17)(q24;q21). A quick APL diagnosis is essential for starting a prompt suitable therapy. We describe a new rapid diagnostic laboratory approach to detect the PML‐RARα rearrangement, which gives clear genetic results within 30 min of hybridization. It combines quick cell harvesting, fluorescence in situ hybridization performed with commercial DNA probe and microwave beams supplied by a domestic microwave oven. Copyright © 2015 John Wiley & Sons, Ltd.