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Overexpression of apoptosis‐associated speck‐like protein in P388D1 murine lymphoma cells affects metastatic properties
Author(s) -
Zhang Yi,
Zhang Jian,
Lin Changwei,
Wei Wei,
Ren Shuangyi,
Zuo Yunfei
Publication year - 2012
Publication title -
hematological oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.918
H-Index - 44
eISSN - 1099-1069
pISSN - 0278-0232
DOI - 10.1002/hon.1010
Subject(s) - apoptosis , transfection , cancer research , spleen , cell culture , metastasis , lymphoma , gene silencing , microbiology and biotechnology , medicine , biology , immunology , cancer , gene , genetics
Apoptosis‐associated speck‐like protein (ASC) is a bipartite adaptor molecule that participates in inflammation and apoptosis. ASC silencing has been observed in a significant proportion of human cancers. Here, we examined the role of ASC overexpression in the metastasis of the P388D1 murine lymphoma cell line to the liver, lung, spleen and kidney. First, we determined that the P388D1 cells express ASC. Then, ASC overexpression in P388D1 was achieved by transfecting pEGFP‐ASC‐C2 into the P388D1 cells. Furthermore, after the ASC‐overexpressing P388D1 cells were injected into DBA/2 mice through the vena caudalis, their metastasis to the lung and the liver was significantly reduced in the pEGFP‐ASC‐C2‐transfected group. These data indicate that ASC overexpression affects the in vivo metastatic properties of P388D1 cells. Copyright © 2011 John Wiley & Sons, Ltd.