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Synthèse par la méthode de Merrifield d'un octapeptide faisant partie du lysozyme de blanc d' œuf de Poule (enchaǐnement Cys 64 → Gly 71 )
Author(s) -
Jollès Pierre,
Jollès Jacqueline
Publication year - 1968
Publication title -
helvetica chimica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.74
H-Index - 82
eISSN - 1522-2675
pISSN - 0018-019X
DOI - 10.1002/hlca.660510438
Subject(s) - chemistry , lysozyme , peptide , edman degradation , residue (chemistry) , peptide synthesis , chromatography , amino acid , peptide sequence , biochemistry , gene
The solid‐phase peptide synthesis of an octapeptide contained in hen egg‐white lysozyme (sequence Cys (residue 64) to Gly (residue 71)) is reported. Each step of the synthesis was verified by amino acid analysis. Three main reaction products were obtained. The octapeptide (50% of the reaction products) was purified by two chromatographies on Dowex 50 × 2. The purified peptide was digested by an aminopeptidase and degradated by means of Edman' s method; this latter procedure has shown that the purified octapeptide still contained around 10% of a shorter peptide.