Premium
Double‐Stranded RNA ‐Specific Templated Reaction with Triplex Forming PNA
Author(s) -
Kim Ki Tae,
Chang Dalu,
Winssinger Nicolas
Publication year - 2018
Publication title -
helvetica chimica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.74
H-Index - 82
eISSN - 1522-2675
pISSN - 0018-019X
DOI - 10.1002/hlca.201700295
Subject(s) - chemistry , rna , dna , oligonucleotide , linker , duplex (building) , nucleobase , nucleic acid , combinatorial chemistry , biochemistry , computer science , gene , operating system
RNA , originally perceived as a simple information transfer biopolymer, is emerging as an important regulator in cellular processes. A number of non‐coding RNA s are double‐stranded and there is a need for technologies to reliably detect and image such RNA s for biological and biomedical research. Herein we report double‐stranded RNA ‐specific templated reaction resulting from PNA ‐reagent conjugates that are brought within reactive distance through the formation of sequence‐specific triplexes onto double‐stranded RNA . The reaction makes use of a ruthenium‐based photocatalyst that reduces a pyridinium‐based immolative linker, unmasking a profluorophore. The reaction was shown to proceed with signal amplification and to be selective for double‐stranded RNA over DNA as well as single‐stranded RNA . The generality of the triplex formation was enabled by non‐canonical nucleobases that extend the Hoogsteen base‐pairing repertoire. The technology was applied to a templated reaction using pre‐micro RNA 31.