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Chemical Synthesis of the 12 kD a Human Myokine Irisin by α ‐Ketoacid‐Hydroxylamine (KAHA) Ligation
Author(s) -
Wucherpfennig Thomas G.,
Müller Sebastian,
Wolfrum Christian,
Bode Jeffrey W.
Publication year - 2016
Publication title -
helvetica chimica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.74
H-Index - 82
eISSN - 1522-2675
pISSN - 0018-019X
DOI - 10.1002/hlca.201600160
Subject(s) - chemistry , myokine , adipose tissue , hydroxylamine , biochemistry , white adipose tissue , receptor , agonist , endocrinology , medicine , skeletal muscle
Irisin is a recently discovered protein hormone with a conserved sequence among vertebrates and with putative functions in the regulation of adipose tissue and bone metabolism. We report the first chemical synthesis using two sequential ketoacid‐hydroxylamine (KAHA) ligations to give milligram quantities of unlabeled and fluorescence‐labeled irisin protein. The synthetic proteins were utilized in cell binding assays, which indicated the expected binding characteristics to stromal cells of white adipose tissue. These studies strongly imply the presence of a specific irisin receptor and provide a path to its identification with synthetic irisin.