Premium
A New Isoflavanol from the Fruits of Kotschya strigosa (Fabaceae)
Author(s) -
Awouafack Maurice D.,
Tchuenguem Roland T.,
Ito Takuya,
Dzoyem Jean P.,
Tane Pierre,
Morita Hiroyuki
Publication year - 2016
Publication title -
helvetica chimica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.74
H-Index - 82
eISSN - 1522-2675
pISSN - 0018-019X
DOI - 10.1002/hlca.201500508
Subject(s) - chemistry , phytochemical , dpph , sephadex , fabaceae , chromatography , ascorbic acid , antioxidant , column chromatography , traditional medicine , botany , food science , organic chemistry , biochemistry , enzyme , biology , medicine
The phytochemical investigation of the MeOH extract from fruits of Kotschya strigosa using repeated normal and reversed‐phase column chromatography and Sephadex LH‐20 chromatography led to the isolation and characterization of a new isoflavanol, named kotstrigoisoflavanol ( 1 ), together with three known compounds, diosmetin ( 2 ), β ‐sitosterol ( 3 ), and the 3‐ O ‐ β ‐d‐glucopyranoside of β‐ sitosterol ( 4 ). The antioxidant activity of crude extract, 1, and 2 was determined using the 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH˙) method. The crude extract ( IC 50 61.7 ± 0.2 μg/ml) and 2 ( IC 50 70.2 ± 0.1 μg/ml) showed moderate antioxidant activities, while 1 was weakly active ( IC 50 153.1 ± 0.1 μg/ml), as compared with the standard reference l ‐ascorbic acid ( IC 50 21.9 ± 0.0 μg/ml).