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Thermal Stability of Modified i‐Motif Oligonucleotides with Naphthalimide Intercalating Nucleic Acids
Author(s) -
ElSayed Ahmed Ali,
Pedersen Erik B.,
Khaireldin Nahid Y.
Publication year - 2016
Publication title -
helvetica chimica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.74
H-Index - 82
eISSN - 1522-2675
pISSN - 0018-019X
DOI - 10.1002/hlca.201500140
Subject(s) - chemistry , oligonucleotide , intercalation (chemistry) , nucleotide , nucleic acid , circular dichroism , cytosine , stereochemistry , nucleobase , crystallography , structural motif , thermal stability , dna , organic chemistry , biochemistry , gene
In continuation of our investigation of characteristics and thermodynamic properties of the i‐motif 5′‐d[(CCCTAA) 3 CCCT)] upon insertion of intercalating nucleotides into the cytosine‐rich oligonucleotide, this article evaluates the stabilities of i‐motif oligonucleotides upon insertion of naphthalimide (1 H ‐benzo[ de ]isoquinoline‐1,3(2 H )‐dione) as the intercalating nucleic acid. The stabilities of i‐motif structures with inserted naphthalimide intercalating nucleotides were studied using UV melting temperatures ( T m ) and circular dichroism spectra at different pH values and conditions (crowding and non‐crowding). This study indicated a positive effect of the naphthalimide intercalating nucleotides on the stabilities of the i‐motif structures compared to the wild‐type structure which is in contrast to a previous observation for a pyrene‐intercalating nucleotide showing a decrease in T m values.