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Identification of Two New Phosphorylated Polyketides from a Brazilian Streptomyces sp. Through the Use of LC – SPE / NMR
Author(s) -
Thomasi Sérgio Scherrer,
Ladeira Cecília,
Ferreira Douglas,
Fontoura Sprenger Ricardo,
Badino Alberto Colli,
Ferreira Antonio Gilberto,
Venâncio Tiago
Publication year - 2016
Publication title -
helvetica chimica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.74
H-Index - 82
eISSN - 1522-2675
pISSN - 0018-019X
DOI - 10.1002/hlca.201500038
Subject(s) - chemistry , polyketide , streptomyces , stereochemistry , combinatorial chemistry , biochemistry , bacteria , biosynthesis , gene , genetics , biology
Two new polyketide phosphate monoesters, phosdiecin A ( 1 ) and phosdiecin B ( 2 ), were isolated from a culture of the marine Streptomyces sp. SS 99 BA ‐2 using the hyphenated technology LC – SPE / NMR . The compounds showed to be new representatives of an important class of antitumor antibiotic metabolites known as fostriecins. Their structures, including relative configuration attribution, were fully elucidated through extensive analyses of NMR and MALDI ‐ TOF / TOF HR‐MS data. Herein, the application of this system to isolate and identify the new compounds is described.