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Identification of Five New Minor Constituents from the Whole Plant of Amischotolype hispida
Author(s) -
Chang ChunWei,
Chang HsunShuo,
Cheng MingJen,
Peng ChienFang,
Chen IhSheng
Publication year - 2015
Publication title -
helvetica chimica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.74
H-Index - 82
eISSN - 1522-2675
pISSN - 0018-019X
DOI - 10.1002/hlca.201400179
Subject(s) - chemistry , antimycobacterial , lignan , dept , metabolite , stereochemistry , bioassay , heteronuclear single quantum coherence spectroscopy , chromatography , mycobacterium tuberculosis , two dimensional nuclear magnetic resonance spectroscopy , biochemistry , tuberculosis , medicine , pathology , biology , genetics
Bioassay‐guided fractionation of the active AcOEt‐soluble layer from the whole plant of Amischotolype hispida resulted in the isolation of four new compounds, i.e. , amisbenzoic acid ( 1 ), one butenolide derivative, amisnolide ( 2 ), one chlorin analog, amisphytin ( 3 ), one lignan, amislignol ( 4 ), and one metabolite isolated for the first time from nature, (−)‐glaberide I ( 5 ), along with 20 known compounds, 6 – 25 . Their structures were elucidated on the basis of UV, IR, 1D‐ and 2D‐NMR ( 1 H, 1 H‐COSY, DEPT, HSQC, HMBC), as well as HR‐ESI‐MS, analyses. Among these isolates, palmitic acid ( 13 ) showed an antimycobacterial activity with an MIC value of 20.0 μg/ml against Mycobacterium tuberculosis H37Rv.