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Diterpenoids from Aerial Parts of Clerodendranthus spicatus and Their Cytotoxic Activity
Author(s) -
Guan ShuCai,
Fan GuangYan
Publication year - 2014
Publication title -
helvetica chimica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.74
H-Index - 82
eISSN - 1522-2675
pISSN - 0018-019X
DOI - 10.1002/hlca.201400106
Subject(s) - chemistry , dapi , terpenoid , cytotoxicity , cytotoxic t cell , two dimensional nuclear magnetic resonance spectroscopy , stereochemistry , terpene , inhibitory postsynaptic potential , indole test , biochemistry , in vitro , apoptosis , neuroscience , biology
A chemical investigation of AcOEt‐soluble portion of the 95% EtOH extract of the aerial parts of Clerodendranthus spicatus led to the isolation and characterization of a new diterpenoid, named clerospicasin J ( 1 ). Structure elucidation of 1 was achieved by spectroscopic analyses, including 2D‐NMR. The diterpenoid 1 exhibited significant inhibitory activity on the proliferation of the SKOV3 cell lines. Moreover, the cytotoxicity of 1 against SKOV3 cells was related to apoptotis as confirmed by DAPI (=2‐(4‐amidinophenyl)‐1 H ‐indole‐6‐carboxamidine) nuclear staining and flow cytometry.