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Clerodendrumic Acid, a New Triterpenoid from Clerodendrum glabrum (Verbenaceae), and Antimicrobial Activities of Fractions and Constituents
Author(s) -
Masevhe Ndivhaleni A.,
Awouafack Maurice D.,
Ahmed Aroke S.,
McGaw Lyndy J.,
Eloff Jacobus N.
Publication year - 2013
Publication title -
helvetica chimica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.74
H-Index - 82
eISSN - 1522-2675
pISSN - 0018-019X
DOI - 10.1002/hlca.201200552
Subject(s) - chemistry , candida albicans , verbenaceae , antimicrobial , chromatography , column chromatography , corpus albicans , traditional medicine , microbiology and biotechnology , biochemistry , organic chemistry , biology , medicine , yeast
One new triterpenoid, (3 β ,11 α ,19 β )‐3‐(butanoyloxy)‐11‐hydroxytaraxast‐20(30)‐ene‐23,28‐dioic acid (clerodendrumic acid; 1 ) was isolated from the hexane extract of the leaves of Clerodendrum glabrum var. glabrum along with heptadecanoic acid ( 2 ). The structure of the new compound was elucidated by interpretation of its NMR (1D and 2D), MS, and IR data. Combined fractions C and D from the column chromatography of the hexane extract exhibited significant antifungal activities (average MIC of 0.10 mg/ml) against Candida albicans and Cryptococcus neoformans. C. albicans was relatively resistant to clerodendrumic acid ( 1 ; MIC 125 μg/ml) and was resistant to heptadecanoic acid ( 2 ; MIC 188 μg/ml). Both compounds had low antibacterial activities against two Gram ‐positive and two Gram ‐negative bacteria with average MIC values of 157 and 172 μg/ml, respectively. Compounds 1 and 2 were relatively nontoxic against monkey kidney Vero cells in vitro with IC 50 values of 202.6 and 108.4 μg/ml, respectively.