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Verification and Biophysical Characterization of a New Three‐Color Förster Resonance‐Energy‐Transfer (FRET) System in DNA
Author(s) -
Altevogt née Kienzler Andrea,
Flehr Roman,
Gehne Sören,
Kumke Michael U.,
Bannwarth Willi
Publication year - 2012
Publication title -
helvetica chimica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.74
H-Index - 82
eISSN - 1522-2675
pISSN - 0018-019X
DOI - 10.1002/hlca.201100460
Subject(s) - förster resonance energy transfer , chemistry , biomolecule , chromophore , fluorescence , ruthenium , energy transfer , resonance (particle physics) , photochemistry , chemical physics , optics , atomic physics , organic chemistry , physics , biochemistry , catalysis
We report on a new three‐color FRET system consisting of three fluorescent dyes, i.e. , of a carbostyril (=quinolin‐2(1 H )‐one)‐derived donor D, a (bathophenanthroline)ruthenium complex as a relay chromophore A 1 , and a Cy dye as A 2 (FRET= Förster resonance‐energy‐transfer) ( cf. Fig. 1 ). With their widely matching spectroscopic properties ( cf. Fig. 2 ), the combination of these dyes yielded excellent FRET efficiencies. Furthermore, fluorescence lifetime measurements revealed that the long fluorescence lifetime of the Ru complex was transferred to the Cy dye offering the possibility to measure the whole system in a time‐resolved mode. The FRET system was established on double‐stranded DNA ( cf. Fig. 3 ) but it should also be generally applicable to other biomolecules.